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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de. |
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Afiliação: |
G. A. F. OLIVEIRA, UFRB; JORGE LUIZ LOYOLA DANTAS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Development and validation of minisatellite markers for Carica papaya. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Biologia Plantarum, v. 59, n.4, p 686-694, 2015. |
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DOI: |
10.1007/s10535-015-0551-9686 |
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Idioma: |
Inglês |
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Conteúdo: |
Whereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. This study clearly demonstrates the polymorphism of minisatellites and their potential use in detection of intraspecific genetic variations in C. papaya. The results will be useful when managing genetic resources and plant breeding. MenosWhereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. ... Mostrar Tudo |
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Thesagro: |
Carica Papaya; Mamão; Variação genética. |
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Thesaurus Nal: |
Genetic variation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02343naa a2200205 a 4500
001 2028333
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s10535-015-0551-9686$2DOI
100 1 $aOLIVEIRA, G. A. F.
245 $aDevelopment and validation of minisatellite markers for Carica papaya.$h[electronic resource]
260 $c2015
520 $aWhereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. This study clearly demonstrates the polymorphism of minisatellites and their potential use in detection of intraspecific genetic variations in C. papaya. The results will be useful when managing genetic resources and plant breeding.
650 $aGenetic variation
650 $aCarica Papaya
650 $aMamão
650 $aVariação genética
700 1 $aDANTAS, J. L. L.
700 1 $aOLIVEIRA, E. J. de
773 $tBiologia Plantarum$gv. 59, n.4, p 686-694, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
25/04/2017 |
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Data da última atualização: |
27/04/2017 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
KLEIN, M. A.; COSTA, F. de S. |
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Afiliação: |
MARCELO ANDRE KLEIN, CNPT; FALBERNI DE SOUZA COSTA, CPAF-Acre. |
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Título: |
Manejo conservacionista do solo e produtividade de milho no Juruá. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
A Tribuna, Rio Branco, 12 ago. 2016. Artigo eletrônico. |
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Idioma: |
Português |
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Conteúdo: |
O milho é uma das culturas mais versáteis na propriedade agrícola, característica que se deve aos seus múltiplos usos. É insumo básico nas cadeias produtivas de suínos e aves e também na bovinocultura de corte e leite, podendo ser utilizado como silagem. Na alimentação humana, pode ser consumido de forma in natura como milho verde ou seco, na elaboração de bolos e outros alimentos. Por fim, pode simplesmente ser comercializado após atingir seu ponto de colheita. No contexto da agricultura familiar, além de representar importante fonte de renda, o milho pode significar uma alternativa para garantir segurança alimentar. |
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Palavras-Chave: |
Acre; Agricultura conservacionista; Amazonia Occidental; Amazônia Ocidental; Calidad del suelo; Cero labranza; Conservación de suelos; Explotación agrícola familiar; Maiz; Manejo del suelo; Regional Juruá; Rendimiento de los cultivos; Rentabilidad agrícola; Western Amazon. |
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Thesagro: |
Agricultura familiar; Manejo do solo; Melhoramento do solo; Milho; Plantio direto; Rendimento; Rentabilidade; Sistema de cultivo; Zea mays. |
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Thesaurus NAL: |
Corn; Crop yield; Family farms; Farm profitability; No-tillage; Soil conservation; Soil management; Soil quality. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/159325/1/26307.pdf
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Marc: |
LEADER 02026nam a2200493 a 4500
001 2068946
005 2017-04-27
008 2016 bl uuuu u00u1 u #d
100 1 $aKLEIN, M. A.
245 $aManejo conservacionista do solo e produtividade de milho no Juruá.$h[electronic resource]
260 $aA Tribuna, Rio Branco, 12 ago. 2016. Artigo eletrônico.$c2016
520 $aO milho é uma das culturas mais versáteis na propriedade agrícola, característica que se deve aos seus múltiplos usos. É insumo básico nas cadeias produtivas de suínos e aves e também na bovinocultura de corte e leite, podendo ser utilizado como silagem. Na alimentação humana, pode ser consumido de forma in natura como milho verde ou seco, na elaboração de bolos e outros alimentos. Por fim, pode simplesmente ser comercializado após atingir seu ponto de colheita. No contexto da agricultura familiar, além de representar importante fonte de renda, o milho pode significar uma alternativa para garantir segurança alimentar.
650 $aCorn
650 $aCrop yield
650 $aFamily farms
650 $aFarm profitability
650 $aNo-tillage
650 $aSoil conservation
650 $aSoil management
650 $aSoil quality
650 $aAgricultura familiar
650 $aManejo do solo
650 $aMelhoramento do solo
650 $aMilho
650 $aPlantio direto
650 $aRendimento
650 $aRentabilidade
650 $aSistema de cultivo
650 $aZea mays
653 $aAcre
653 $aAgricultura conservacionista
653 $aAmazonia Occidental
653 $aAmazônia Ocidental
653 $aCalidad del suelo
653 $aCero labranza
653 $aConservación de suelos
653 $aExplotación agrícola familiar
653 $aMaiz
653 $aManejo del suelo
653 $aRegional Juruá
653 $aRendimiento de los cultivos
653 $aRentabilidad agrícola
653 $aWestern Amazon
700 1 $aCOSTA, F. de S.
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